2023-03-20
This is Stephanie's daily open-notebook.
Today is 2023.03.20
Todo today
Have a look at the DBGI discussion forum
- https://github.com/orgs/digital-botanical-gardens-initiative/discussions
sample code
blank STG03 QC anacardia QC Pseudos QC KLA STG-01-01C STG-01-02C STG-01-03C STG-01-04C STG-01-05C STG-01-06C STG-01-07C STG-01-08C STG-01-09C STG-01-10C STG-01-11C STG-01-12C STG-01-13C STG-01-14C STG-01-15C STG-01-16C STG-01-17C STG-01-18C STG-01-19C STG-01-20C STG-01-21C STG-01-22C STG-01-23C STG-01-24C STG-01-13A STG-01-14A STG-01-15A STG-01-16A STG-01-13B STG-01-14B STG-01-15B STG-01-16B STG-01-025 STG-01-026 STG-01-027 STG-01-028 STG-01-029 STG-01-030 STG-01-031 STG-01-032 STG-01-033 STG-01-034 STG-01-035 STG-01-036 STG-01-037
Doing
Paused
Done
- on 17/03/2023 I orked with Eliane on the Agilent 1260 HC-DAD to develop a method to analyse the anacardiaceae samples. we took as a basis a method I previously used to analyse the leaves extracts of P. microcarpa. After several attempts we adopted the method below which gave rise to a better separation -T= 0 min 30% B 70% A -T= 1 min 30% B 70% A -T= 25 min 60 % B 40% A -T= 28 min 100% B 0% A -T= 33 Min 100% B 0% A -T= 38 min 30% B 70 % A for a Total run time 45 min where B is methanol containg 0.1 % TFA and A is water containing 0.1 % TFA. injection volume 10 Ul flow rate 0.2 mL/min DAD dectector at 360nm, 275 nm, 245 nm and 307 nm.
I have prepare the injection list for the batch odf sample to be analysed.
Notes
We used the method above to analyse pseudopondias samples 11 samples + seven pure compounds previously isolated from the leaves of Pseudospondias.