Expanding Pfas Identification With Transformation Product Libraries
Expanding PFAS Identification with Transformation Product Libraries: Nontargeted Analysis Reveals Biotransformation Products in Mice
Terms
- Exposome: The complete set of environmental exposures experienced by an organism throughout its lifetime.
- PFAS: Per/polyfluroakyl substances (fluorinated substances) that persist in nature and are the result of manufacturing.
- Transformation product: (TP) Molecule produced when another molecule is modified.
- Biotransformation: Transformation of chemical in a biological system.
- Phase I and II metabolism: Phase 1: metabolic reactions that modify a molecule. Phase 2: Reactions that conjugate molecules with groups like sulfates.
- Sulfation: Phase 2 metabolic pathway that attaches sulfate group to molecule.
- In Vitro: performed outside of organism (like in a test tube)
- Nontargeted analysis: (NTA) mass spec method that searches for unknown compounds
- Fragmentation rule: The rules/patterns used to identify chemicals
- Kendrick mas defect (KMD): transformation used to identify homologous series in MS/MS
- Molecular feature: Ion feature representing potential compound
- Toxicokinetics: study of chemical absorption and metabolization
Results
They evaluated 27 different PFAS, and they were able to find some parent PFAS (10) and 14 transformation products. And they were able to find 4 biological pathways for PFAS transformation.
Conclusions
Measuring the parent transformations is important for identifying PFAS contamination and pathways in bio systems. They expanded the fluoromatch library